Uji Aktivitas Antioksidan beberapa Fraksi Ekstrak Fuli Pala (Myristica fragrans Houtt) Dengan metode DPPH

Abstract

This study aims to evaluate the antioxidant activity of n-hexane fraction, chloroform fraction, and ethyl acetate fraction of Nutmeg (Myristica fragrans) mace extract using DPPH method. The results showed that the chloroform fraction had the highest antioxidant activity with an IC₅₀ value of 22.17, close to the effectiveness of Vitamin C which was used as a positive control with an IC₅₀ value of 18.60. The n-hexane fraction showed moderate antioxidant activity with an IC₅₀ value of 71.57, while the ethyl acetate fraction had the lowest activity with an IC₅₀ value of 129.89. This data suggests that the chloroform fraction of the nutmeg mace extract has the greatest potential as a natural antioxidant. The yield of the ethyl acetate fraction was the highest (40%), followed by the n-hexane fraction (38%) and chloroform fraction (10.8%). This study provides important insights into the effectiveness of various fractions of Mace extract in counteracting free radicals and opens up opportunities for therapeutic or nutraceutical applications. These findings support the use of nutmeg mace as a potential source of natural antioxidants, especially the chloroform fraction, in efforts to prevent oxidative stress-related diseases.