The Effect of Incubation Time on The Quality of Sexing Simmental Cattle Semen with The BSA (Bovine Serum Albumin) Column Method

Abstract

Simmental has the advantage of good reproduction and meat production. In addition, Simmental cow semen has a better viscosity level than other cow semen, so it can be used in spermatozoa sexing technology. One method of sex separation of bovine spermatozoa is the BSA column method which is able to produce 75-80% Y sperm. This study aims to determine the quality of fresh Simmental bovine semen (macroscopic and microscopic tests) and sexing (motility, viability and proportion tests). The results of the macroscopic test of Simmental cattle's fresh semen in general, the cement used in this study had good quality. The color of normal cow semen has a yellowish white color. The smell of cow semen has a distinctive smell. The volume of semen in this study was 6 ml, medium consistency, and pH 6.8. The results of the microscopic test had a mass movement of 2 (++), a motility of 70 and a concentration of 1650±259.81. The motility of the X spermatozoa sexing results, the best incubation time is 45 minutes, the motility is 55.00±5.00, while the motility of the Y spermatozoa sexing results is the best 30 minutes, the motility is 45.00±11.18. The viability of the sexed X spermatozoa was 54.85±10.25 with an incubation time of 45 minutes, while the best sexed Y spermatozoa was 32.6±13.98 with an incubation time of 30 minutes..